The 44-year-old, whose real name is Marshall Mathers, made the blunder while performing smash hit single Stan at Glasgow Summer Sessions at Bellahouston Park last night.
In a clip, filmed by fan Robbie Loughlin, Eminem can be can be heard rapping: “I read about your Uncle Ronnie too, I’m sorry.
“I had a friend kill himself over some b***h who didn’t want him.”
The star then stumbles his words, goes silent and looks behind him at his crew while letting out a loud sigh and laughing.
He then signals the sound team saying “You gotta run that again” before managing to pick up the song and continuing right through until the end.
The blunder was met with cheers from the crowd who, during the gig, he claimed were the “best crowd he had ever had”.
Since his appearance last night, footage has started circulating over social media showing Eminem’s mistake.
Hundreds of rap fans have commented on clips with many praising him for carrying on.
Helen Last wrote: “He did the right thing laughed with the crowd and carried on like a pro still did a great concert.”
Marcus Garcia said: “Man, Eminem can take anything!”
And Kristen Taylor wrote: “We all mess up, and Eminem is still awesome as f***.”
One fan Jay Marie, had his own theory about the blunder, referring to Eminem’s new facial hair, writing: “The beard is f****** with his lyrics.”